https://doi.org/10.1140/epjs/s11734-024-01378-x
Regular Article
Assessment of OTR measurement techniques in the bubble environment of an industrial fermenter
1
Laboratory of Multiphase Physical and Biological Media Modeling, Ural Federal University, Ekaterinburg, Russia
2
Moscow Institute of Physics and Technology, Moscow, Russia
3
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
4
NPO Biosintez Ltd., Moscow, Russia
5
Boreskov Institute of Catalysis, Novosibirsk, Russia
6
Altai State University, Barnaul, Russia
7
Otto Schott Institute of Materials Research, Friedrich Schiller University, Jena, Germany
Received:
2
September
2024
Accepted:
17
October
2024
Published online:
14
November
2024
A comprehensive assessment of oxygen transfer rate (OTR) measurement techniques in the bubble environment of large-scale industrial fermenters is presented. Specifically, we focus on the sodium sulfite and hydrogen peroxide methods, two of the most commonly used approaches for measuring OTR in high oxygen-demand systems, and compare them with the nitrogen degassing method. A theoretical review of these methods is conducted and supplemented with experimental data collected from a 5-l stirred-tank fermenter. Through this comparative analysis, the key differences between the methods are highlighted, including the enhancement factor effect, their applicability to industrial bioreactors with high volumetric mass transfer coefficients (
), and the challenges associated with scaling these techniques to industrial systems. Results underscore the critical role of OTR in determining mass transfer efficiency in aerobic bioreactors, while also outlining the relationship of OTR to biochemical reactions in the environment.
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© The Author(s), under exclusive licence to EDP Sciences, Springer-Verlag GmbH Germany, part of Springer Nature 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
