https://doi.org/10.1140/epjst/e2012-01676-1
Regular Article
Nano-structured cellulose nanocrystals-xyloglucan multilayered films for the detection of cellulase activity
1 INRA, UR1268 Biopolymères Interactions Assemblages, 44316 Nantes, France
2 Centre de Recherches sur les Macromolécules Végétales (CERMAV-CNRS), BP. 53, 38041 Grenoble Cedex 9, France
3 Laboratoire Léon Brillouin, CEA/CNRS Saclay, 91191 Gif-sur-Yvette Cedex, France
4 Institut de Physique de Rennes, CNRS-UMR 6251, Université de Rennes 1, 35042 Rennes, France
a e-mail: aurelieguyomard@gmail.com
Received:
20
July
2012
Revised:
27
September
2012
Published online:
3
December
2012
Multilayered cellulose nanocrystals-xyloglucan (CN-XG) films were assembled by spincoating at two CN concentrations inducing different structural colourations. Internal structure of both films was probed by neutron reflectometry (NR) revealing different thicknesses and architectures. The impact of film structure on degradation process by cellulases was revealed by a change in colours of the films, as CN and XG are both sensitive to cellulolytic enzymes.
© EDP Sciences, Springer-Verlag, 2012
