https://doi.org/10.1140/epjst/e2009-00956-1
Characterizing the structure of protein layers adsorbed onto functionalized surfaces by means of in-situ X-ray reflectivity
1
Fakultät Physik/DELTA, TU Dortmund, Maria-Goeppert-Mayer-Str. 2, 44221 Dortmund, Germany
2
Fakultät Chemie, TU Dortmund, Otto-Hahn-Str. 6, 44221 Dortmund, Germany
Corresponding author: florian.evers@tu-dortmund.de
In a biological cell, proteins face a highly complex environment comprising crowding and confinement effects as well as interactions with interfaces, cosolvents, and other biomolecules. In this study, the X-ray reflectivity technique has been used for the in-situ characterization of adsorbed protein layers at solid-liquid interfaces. The adsorption of bovine serum albumin at the hydrophobic polystyrene-water interface has been investigated in the presence and in the absence of salts. The data indicate that enhanced adsorption occurs at high salt concentrations.
© EDP Sciences, Springer-Verlag, 2009
